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Histochemical Reagents

Aniline Blue Fluorochrome for callose and related (1-3)-beta-glucans
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An analytical probe for (1-3)-beta-D-glucans (callose). Highly specific for (1-3)-beta-glucans [Evans et al. (1984) Carbohyd. Polymers 4: 215-230; Stone et al. (1984) Protoplasma 122: 191-195]. May be used for the quantitative determination of callose [Kauss (1989) Plant Physiol. 81: 171-176] and in fluorescence assays for (1-3)-beta-glucan synthase products.

Yariv Reagents for arabinogalactan-proteins (AGPs)
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Specific probes for the detection of AGPs in tissue sections [Anderson et al. (1977) Aust J. Plant Physiol. 4: 143-158]. May be used to detect and quantify AGPs in tissue extracts [Van Holst & Clarke (1985) Anal Biochem. 148: 446-450] and to detect AGPs in crossed-electrophoretic separations [Van Holst & Clarke (1986) Plant Physiol. 80: 786-798].

Gum Arabic an arabinogalactan-protein
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Gum Arabic AGP is used as a reference standard in methods involving detection, quantification and analytical analyses of AGPs from plant tissues.

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Immunohistochemical Reagents

Monoclonal Antibodies (Murine)

to (1-3)-beta-D-glucan
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No cross-reactivity with (1-4)-beta-D-glucans or (1-3;1-4)-beta-D-glucans [Meikle et al. (1991) Planta 188: 1-8].

to (1-3;1-4)-beta-D-glucan
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No cross-reactivity with (1-3)-beta-D-glucans [Meikle et al. (1994) The Plant Journal 5: 1-9].

to (1-4)-beta-D-mannan and galacto-(1-4)-beta-D-mannan
[Pettolino et al. (2001) Planta 214: 235-242]
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Each of these antibodies can be used with second stage, gold- or fluorochrome-labelled rabbit, anti-mouse antibody for immunohistochemical studies.

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Enzymes

(1-3;1-4)-beta-D-glucan Hydrolase from Bacillus subtilis (EC 3.2.1.73)
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Specifically hydrolyses beta-D-glucans containing both (1-3)- and (1-4)-beta-D-glucosidic linkages in linear sequences. Does not hydrolyse (1-4)-beta-D-glucans or (1-3)-beta-D-glucans [Anderson & Stone (1975) FEBS Letters 52: 202-207].

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Substrates

Pachyman [(1-3)-beta-D-glucan] ex Poria cocus
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Useful as a positive control in fluoroescence microscopy studies on callose using the aniline blue fluorochrome or (1-3)-beta-D-glucan specific monoclonal antibody.

Arabino-(1-4)-beta-D-xylan from wheat flour
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A water-soluble polysaccharide from walls of wheat endosperm cells. Useful as a substrate for (1-4)-beta-D-xylan hydrolase.

Uridine diphospho (13C6) glucose
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As a substrate for in vitro synthesis of glucans allowing rapid and detailed characterisation of the products using 13C NMR.

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Reference Text

Chemistry and Biology of (1-3)-beta-glucans

By Bruce A Stone and Adrienne E Clarke

Glucans, with the (1-3)-beta-glucosidic linkage as a major feature, are present in most higher plants and many lower plants and microorganisms. They may occur as major structural or storage components or be formed at very specific sites in response to particular developmental events or stimuli. In many cases their functional role is a mystery, in others it is well established. Their distribution and physiological involvement indicates that they are important to fields such as agriculture and biotechnology, and may also have impact in medicine, throught their role in immunology and cancer therapy.

Contents:

Structural characterisation and physical chemistry of:

  • (1-3)-beta-glucans
  • Enzyme depolymerising (1-3)-beta-glucans
  • Biosynthesis of (1-3)-beta-glucans
  • Prokaryote (1-3)-beta-glucans
  • Algal (1-3)-beta-glucans
  • Fungal, yeast and lichen (1-3)-beta-glucans
  • Chemistry and physiology of higher plants (1-3)-beta-glucans (callose)
  • (1-3)(1-4)-beta-glucans in higher plants
  • (1-3)-beta-glucans in plant host-pathogen interactions
  • (1-3)-beta-glucans and (1-3)-glucan hydrolases in animals
  • (1-3)-beta-glucans and animal defence mechanisms

References and index

The book is an invaluable reference handbook not only for graduate students and teachers in universities but also for scientists in industrial, agricultural and medical research and develpment laboratories.

Special features of 'Chemistry and Biology of (1-3)-beta-glucans' include:

  • Hard cover & section sewn binding
  • 808 pages
  • Over 150 tables and illustrations
  • Exhaustive index of over 50 pages
  • First published 1992

USD$91 plus postage and handling

.................................................................................

Chemistry, Biochemistry, and Biology of (1-3)-beta-Glucans and Related Polysaccharides

By Antony Bacic, Geoffrey B. Fincher, Bruce A. Stone

Key Features:

  • Topics of medical relevance include detailing the glucans' interactions with the immune system and research for cancer therapy applications
  • Web resource links allow scientists to explore additional beta glucan research
  • Separate indexes divided into Species and Subject for enhanced searchability

Description:

The book presents a comprehensive, systematic and authoritative survey of information about a family of chemically related, but functionally diverse, naturally occurring polysaccharides- the (1-3)-glucans. International contributors describe the chemical and physicochemical properties of these glucans and their derivatives and the molecular biological and structural aspects of the enzymes involved in their formation and breakdown. A detailed analysis of their physiological roles in the various biological situations in which they are found will be provided. Additionally, evolutionary relationships among the family of these glucans will be described.

[For more information on this book, click here.]

 

Pub. Date: August 2009
List Price: $125 / € 91.95 / £62.99
ISBN-13: 978-0-12-373971-1
Audience: Plant physiologists & cell biologists, enzymologists, polysaccharide chemists, immuno-chemists, immunologists, alternative cancer therapy researchers

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